Figure 6.

PARG activity is essential for prostate cancer cell proliferation and survival during BER challenge. LNCaP cells were plated onto E-plates in media containing FBS (a) or CSS (b) and allowed to adhere overnight. The following day cells were treated with either DMSO vehicle, 1 µM of PDDX, 100 µM temozolomide, or a combination of 1 µM PDDX and 100 µM temozolomide. Cell proliferation during the next 40 hours was monitored using the RTCA system. (c,d) LAPC4 cells were plated into E-plates in the presence of media containing FBS + R1881 (c) or CSS (d) and allowed to adhere overnight. The following day cells were treated with the same concentration of compounds as LNCaP cells in (a,b). Cell proliferation during the next 30 hours of treatment was monitored using the RTCA system. For figures (a–d), p values for the final time point are shown under the graph. Each point on the panels a-d represents an average of 4 biological replicates and standard error of the mean. The experiments were performed 3 times. (e,f) LNCaP cells were plated in FBS medium (e) or CSS medium (f) at a density of 2 × 10³ cells per/well. Cells were treated with DMSO vehicle, 1 µM PDDX, 100 µM temozolomide, or a combination of 1 µM PDDX and 100 µM temozolomide for 48 hours. Following treatment, cell viability was determined using the MTT assay. (**p ≤ 0.01; ***p ≤ 0.001). Each bar is an average of 4 biological replicates, experiments were performed 3 times.