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. 2020 Mar 2;10:3791. doi: 10.1038/s41598-020-60185-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Resveratrol switched M1 to M2 macrophages. (a) Representative flow cytometry plots and the sequential gating strategy for CD11c and CD206 markers in CD11b and F4/80 dual positive cells for analysis of macrophages polarization in skeletal muscle tissue of NCD, HFD and HFD + RES groups. (b) Percentage of CD11c⁺ cells and CD206⁺ cells in total macrophages. (c) iNOS and CD11c (M1 markers) mRNA expression in skeletal muscle tissue. (d) CD206 and arginase1 (M2 markers) mRNA expression in skeletal muscle tissue. All data were analyzed by one-way ANOVA, Tukey post-test Values are expressed as means ± SD *p < 0.05, **p < 0.01, ***p < 0.001 vs. the control group. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. the HFD group.