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. 2020 Mar 2;10:3791. doi: 10.1038/s41598-020-60185-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative western blot is shown. Phosphorylated p38, total p38, phosphorylated JNK, total JNK, phosphorylated AMPK, total AMPK and NF-κB (p65), and control β-Actin levels were evaluated by immunoblot analysis. Protein bands were quantified by densitometry and normalized to β-Actin levels. Full-length blots are presented in Supplementary Fig. 3. All data were analyzed by one-way ANOVA, Tukey post-test. Data are presented as means ± SD *p < 0.05, **p < 0.01, ***p < 0.001 vs. the control group. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. the HFD group.