Skip to main content
. 2020 Mar 2;11(3):160. doi: 10.1038/s41419-020-2316-4

Fig. 4. HMGA1 regulates autophagy in NRCMs.

Fig. 4

a–c Protein levels of LC3, P62, and Atg7 in NRCMs transfected with Ad-HMGA1 (n = 6). d NRCMs were transfected with mRFP-GFP-LC3-adenovirus and Ad-HMGA1 and then stimulated with HG for 48 h. mRFP and GFP fluorescence and quantitated yellow puncta number per cell (n = 5). *P < 0.05 vs. the Ad-NC-CON group; #P < 0.05 vs. the Ad-NC-HG group. e–g Western blot image and quantification of LC3, P62, and Atg7 in NRCMs transfected with HMGA1 siRNA (n = 6). h NRCMs were transfected with mRFP-GFP-LC3-adenovirus and HMGA1 siRNA and then stimulated with HG for 48 h. mRFP and GFP fluorescence and quantitated red, green, yellow puncta number per cell (n = 5). *P < 0.05 vs. the ScRNA-CON group; #P < 0.05 vs. the ScRNA-HG group. All the in vitro experiments were performed independently for three times.