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. 2020 Mar 2;11(3):160. doi: 10.1038/s41419-020-2316-4

Fig. 5. Autophagy mediates the functional role of HMGA1 in cardiomyocytes.

Fig. 5

NRCMs were transfected with HMGA1 siRNA, Atg5 siRNA, treated with bafilomycin A1 (Baf) for 2 h and then stimulated with HG for 48 h. NRCMs were transfected with Ad-HMGA1, Ad-Atg5, and treated with rapamycin (RAP) for 2 h, and then stimulated with HG for 48 h. a The expression level of Atg5 when cells were transfected with Atg5 siRNA or Ad-Atg5 (n = 6). b NRCMs were transfected with mRFP-GFP-LC3-adenovirus. mRFP and GFP fluorescence and quantitated red, green, yellow puncta number per cell (n = 5). c Transcription levels of proinflammatory markers in NRCMs in the indicated group (n = 6). d, e TUNEL staining and quantification results in NRCMs in the indicated groups (n = 5). *P < 0.05 vs. the CON group; #P < 0.05 vs. the HG group. All the in vitro experiments were performed independently for three times.