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. 2020 Mar 2;11(3):160. doi: 10.1038/s41419-020-2316-4

Fig. 6. HMGA1 regulates P27/CDK2/mTOR signaling.

Fig. 6

a, b, e Western blot image and quantification of phosphorylated (P-) and total (T-) AMPKα, mTOR, 4EBP1, AKT, ERK1/2, P27, and CDK2 in NRCMs transfected with Ad-HMGA1 (n = 6, *P < 0.05 vs. the Ad-NC-CON group; #P < 0.05 vs. the Ad-NC-HG group). c, d, f Western blot image and quantification of phosphorylated and total AMPKα, mTOR, 4EBP1, AKT, ERK1/2, P27, and CDK2 in NRCMs transfected with HMGA1 siRNA (n = 6, *P < 0.05 vs. the ScRNA-CON group; #P < 0.05 vs. the ScRNA-HG group). All the in vitro experiments were performed independently for three times.