Table 1.

Primers used for qPCR

Gene	Protein	Primer sequence		Amplicon length (bp)	Efficiency (%)
		Forward	Reverse
CCSER2	Coiled-coil serine-rich protein 2	TGAGCAGAGGCTCTCCCTAT	GGAAAAAGGGCATCCCTCCA	72	99.5
HK1	Hexokinase 1	AGCACTGCTCCAGGTCCGGGCT	GCGGCCCTCCTGGACACCACCC	108	101.2
HK2	Hexokinase 2	GCCTGGCTAACTTCATGG	GAGCCCATGTCAATCTCC	361	104.2
HTR1A	5-hydroxytryptamine receptor 1A	CGAATCTTCGCGCTGCTTTT	TGATGTGGTGTTGTTGCCCT	97	97.3
HTR1B	5-hydroxytryptamine receptor 1B	CCCTGGAAAGTACTGCTGGT	TCCGGTACACTGTGGCAATC	91	96.8
HTR1D	5-hydroxytryptamine receptor 1D	CTCACCAGGAAGCTCCACAC	TGGAAACCAAGAGGTCGGTG	73	98.3
HTR1E	5-hydroxytryptamine receptor 1D	CGCACCGTCCACAAGGAAC	GTGGTCTCTTGTGTTCCATTTTCT	84	95.4
HTR1F	5-hydroxytryptamine receptor 1F	AGTGGGCCCTAGTGAAGGTAT	TCTGAGGTCAAGTTTTGATCAGATG	100	97.8
HTR2A	5-hydroxytryptamine receptor 2A	CCTGTATGGGTACCGGTGG	GATGGAGGCCGTGGAGAAG	85	101.1
HTR2B	5-hydroxytryptamine receptor 2B	TGGTGTGGTTAATTTCAATAGGCAT	TGGGTTGTCCACATCAGTCTC	74	99.3
HTR2C	5-hydroxytryptamine receptor 2C	TCCTTTATGATTATGTCTGGCCACT	GTGCATGATGGACGCTGTTG	92	95.9
HTR3A	5-hydroxytryptamine receptor 3A	TGCTCAGCCATGGGAAACC	TACATCTGTCCCTCGGGCTC	76	96.5
HTR3B	5-hydroxytryptamine receptor 3B	GGTAACAGAGTCCTCGCTGTATG	TGATAGATTGAAGCTGCGACCA	83	98.8
HTR3C	5-hydroxytryptamine receptor 3C	GGCCACCCCAAAGATGTCC	GCGCCTGATGGCCACATAA	70	102.1
HTR3D	5-hydroxytryptamine receptor 3D	CTCCCAGCCACTAGCACTTC	GCGAAGTAGACACCTCGCTT	83	100.8
HTR3E	5-hydroxytryptamine receptor 3E	GCAAGGCCACCGCAAAG	CTGATGGCCACATAGAACACGA	70	99.2
HTR4	5-hydroxytryptamine receptor 4	GACAGGCAGCTCAGGAAAATAAA	CAATGGCACCAAAGGGCATC	100	97.2
HTR5A	5-hydroxytryptamine receptor 5A	TCTACAAGGCTGCCAAGTTCC	GTCCTTCACCTCCACAGCTT	83	95.9
HTR6	5-hydroxytryptamine receptor 6	CATAGTCCAGGCCGTGTGC	ACAGTAACCCAGCCATGTGAG	70	99.7
HTR7	5-hydroxytryptamine receptor 7	GAGACCTGAGTTTGTGCTGAGG	ATACCGGTGGCCTCTTTTCTG	71	96.9
LDHA	Lactate dehydrogenase A	GGCACGTCAGCAAGAGGGAGA	GCAACTTGCAGTTCGGGCTGT	110	97.8
LDHB	Lactate dehydrogenase B	ACACCGCGTGATTGGAAGTGGA	TGGCAGCTGCTGGGATGAATGC	93	96.4
PDK1	Pyruvate dehydrogenase 1	TGCTTCGCGGAGCCGCCTTGG	CGCTCGGACGCCGGGCTGGA	103	95.0
PFKM	PFK isoform M	GGAGTGCGTGCAGGTGACCAAA	ATCACGGCCACTGTGTGCAACC	171	99.3
PFKL	PFK isoform L	CATGAATGCAGCTGTGCGCTCC	CCAGCCCACTTCTTGCACCTGA	118	102.6
PFKP	PFK isoform P	ACAGACACGTGCGACCGCAT	AGTGCACGACGTTGGACTGCA	187	98.5
PKL	PK L	TTGGGCGCTGCAACTTGGCGGG	CTGCCCTCGTTGGCCGGGGCT	90	97.7
PKM1	PK M1	GGCTGAGGACGTGGACCTCCGGG	AGCCAGGGCGCCATCCGGTCA	110	96.1
PKM2	PK M2	GCAAGGCATCTGATGTCC	CCCTTTGGCTGTTTCTCC	368	97.8
SLC16A1	MCT1	GTCATTGGAGGTCTTGGGCT	GCCAATGGTCGCCTCTTGTA	86	98.8
SDHA	SDH A	TGGGGAGTGCCGTGGTGTCA	TAGGTGCGCCCGTAGCCTCC	105	99.7
SIRT3	SIRT3	CCTGGAGGTGGAGCCTTTTG	AAGTCCCGGTTGATGAGCAG	78	101.1

All the primers were optimised for 600 nM use in the experiments