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. 2020 Mar 2;11:1147. doi: 10.1038/s41467-020-14936-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Somatic ovarian cortical cells expressing DDX4 were manually pooled for analysis of transcriptional profile. a Heatmap showing representative marker genes of OSCs and pluripotent cells by cell cluster. b Feature and violin plots highlighting all somatic cells expressing DDX4 transcript. Expression is mainly localized to the oocyte cluster (pink circle in feature plot) and dispersedly in 15 cells from other clusters (labeled with * for clarity). The 15 somatic cells expressing DDX4 were manually selected to form their own cluster (ddx4* in violin plot). c Violin plots displaying the expression scores of reported OSC marker genes (DPPA3, DAZL, PRDM1), germline and/or pluripotency-associated genes (POU5F1, NANOG, TFAP2C) and oocyte marker genes (GDF9, ZP3, OOSP2, FIGLA) across all cell clusters. Ddx4* cluster does not show differential expression of these markers. The horizontal bars in violin plots indicate median gene expression per cluster. G granulosa, I immune, O oocytes, UMAP uniform manifold approximation and projection.