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. 2020 Feb 25;11:143. doi: 10.3389/fpls.2020.00143

Figure 1.

Figure 1

hda9 mutants are insensitive to ABA. (A) Seeds of WT, hda9-1, hda9-2, and hda9-1/HDA9 were germinated on MS media supplemented with and without 5 μM ABA. Photographs were taken 5 d after sowing. (B) Quantification of germination rate of WT, hda9-1, hda9-2, and hda9-1/HDA9 on different concentrations of ABA. The values indicated means ± SE of n = 4 biological replicates of at least 32 seeds for each experiment. Asterisks represent significant differences from the WT (*, 0.01 < p-value ≤ 0.05; **, p-value < 0.01; Student's t-test). (C) ABA-induced stomata closure. Leaves of 10-d-old seedling of WT, hda9-1, hda9-2, and hda9-1/HDA9 were floated in stomatal opening solution for 2 h and then incubated in the 5 μM ABA for 2 h in the light. Stomata on the abaxial surface were observed using scanning electron microscopy. (D) Measurement of stomatal aperture (the ratio of width to length) after ABA treatment. The values indicated means ± SE of n = 4 biological replicates of 50 to 80 stomata for each experiment. Asterisks represent significant differences from the WT (*, 0.01 < p-value ≤ 0.05; Student's t-test).