Figure 6.

HDA9 is associated with the promoters of CYP707A1 and CYP707A2 in dehydration response. The ChIP assays of the CYP707A1 and CYP707A2 chromatin regions associated with HDA9. The ChIP assays were performed on nuclear proteins extracted from two-week-old seedling hda9-1/HDA9 (with HA tag) plants with/without dehydration stress. Chromatin complex was immunoprecipitated with anti-HA antibody. Samples were quantified by real-time qPCR using specific primers for the amplicons on the different regions of the CYP707A1 and CYP707A2 promoter. Schematic drawing of the CYP707A1 (at the top of A) and CYP707A2 (at the top pf B) locus and locations of the ChIP assay amplicons (A1-1 to A1-3, A2-1 and A2-2). TUBULIN4 expression was assessed as the internal control. The ChIP results were presented as fold enrichment of nontarget DNA. Bars represent mean ± SD of four biological replicates with three technical replicates each. Asterisks represent significant differences from the WT (**, p-value < 0.01; Student's t-test).