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. 2020 Feb 25;11:148. doi: 10.3389/fimmu.2020.00148

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Copyright © 2020 Tao, Chen, Wang, Chen, Zhao, Zheng and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Liver fibrosis in WT and CD47KO mice fed LFD or HFD. (A) Liver fibrosis determined by Sirius Red staining. Three samples per group were examined, and representative images are shown. (B) Collagen content determined by counting positive areas in six randomly selected fields using Image Pro Plus 6.0 software. Data from a representative of three independent samples are shown. (C,D) Relative mRNA expression levels of TGFβ (C) and thrombospondin 1 (TSP1) (D), which were determined by quantitative real-time PCR and normalized to β-actin (n = 4 per group). Data are presented as mean ± SD. ^**p < 0.01; ^***P < 0.001; and ^****P < 0.0001.