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. 2020 Feb 25;11:148. doi: 10.3389/fimmu.2020.00148

Figure 4.

Figure 4

CD47 deficiency promotes HFD-induced angiogenesis with upregulation of CD31 and VEGFR in the liver. (A) Representative images of liver sections stained for CD31 (red), TIE2 (green), and DAPI. Merged images are shown in the last column (CD31+TIE2+ cells are indicated by arrows). Data from a representative of three samples are shown. Scale bar represents 50 μm. (B,C) Relative mRNA expression levels of CD31 (B) and VEGFR1 (C) quantified by real-time PCR and normalized to β-actin (n = 4 per group). Data are presented as mean ± SD. ****P < 0.0001.