Figure 5.

CD47 deficiency enhances HFD-induced inflammation and monocyte/macrophage infiltration in the liver. (A–E) Relative mRNA levels of IL-1β (A), IL-6 (B), IL-10 (C), TNFα (D), and C-C motif ligand 2 (CCL2) (E). Gene expression levels were determined by quantitative real-time PCR and normalized to β-actin (n = 4 per group). Data are presented as mean ± SD. ^*p < 0.05; ^**p < 0.01; ^***P < 0.001; and ^****P < 0.0001. (F) Liver sections were immunohistochemistry (IHC)-stained for CCL2. Six samples per group were examined, and representative images are shown (scale bar represents 20 μm). (G) Liver sections were immunofluorescence-stained by anti-CD68 (green) plus DAPI (blue). Four samples per group were examined, and representative images are shown (scale bar represents 50 μm).