Figure 10.

EA, EB, and IEA decrease mHtt-induced cytotoxicity in HT22 cells. (A) HT22 cells transfected with EGFP-HTT74 were treated with EA, EB and IEA at the indicated concentrations for 24 h. After treatment, cells were trypsinzed and stained with PI solution for 15 min, then the cells were analyzed by flow cytometry using the PE channel. Bar chart indicates the percentage of dead HT22 cells; bars, S.D. ^###P ≤ 0.001 VS. the control group. *P ≤ 0.05, VS. the EGFP-HTT74 group. (B) HT22 cells transfected with EGFP-HTT74 were treated with EA, EB and IEA at the indicated concentrations for 24 h. After treatment, cell proteins were harvested for the analysis of Bax, Bcl-2, activated caspase 3, activated caspase 9, and β-actin. Bar chart indicates the relative expression of Bax to Bcl-2, activated caspase 3, and activated caspase 9 to β-actin; bars, S.D. ^#P ≤ 0.05 VS. the control group. *P ≤ 0.05, **P ≤ 0.01 VS. the EGFP-HTT74 group. The full-length Western blot images are showed in Figure S18.