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. 2020 Feb 25;11:116. doi: 10.3389/fphar.2020.00116

Figure 2.

Figure 2

Safe concentrations of ES, EA, EB and IEA in HT22 cells. (A, B) HT22 cells were treated with ES (1.5625, 3.125, 6.25, 12.5, 25, 50, 100, and 200 μg/mL) or EA, EB and IEA (1.25, 2.5, 5, 10, 20, 40, and 80 μM) for 24 h, the cell viability was then detected by using a CCK-8 assay kit. (C, D) HT22 cells were treated with ES (1.5625, 3.125, 6.25, 12.5, 25, 50, 100, and 200 μg/mL) or EA, EB, and IEA (1.25, 2.5, 5, 10, 20, 40, and 80 μM) for 24 h, the LDH release was then assayed by a LDH cytotoxicity detection kit. (E) HT22 cells were treated with ES, EA, EB, and IEA at the indicated concentrations for 24 h, the cell viability was then analyzed by flow cytometry using an annexin V-FITC/PI kit. (F) Bar chart indicates the cell viability of HT22 cells that analyzed by flow cytometry.