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. 2020 Feb 25;11:116. doi: 10.3389/fphar.2020.00116

Figure 9.

Figure 9

EA, EB, and IEA enhance the clearance of mHtt via ATG7. ATG7+/+ MEF and ATG7–/– MEF cells transfected with EGFP-HTT74 were treated with ES (A), EA (B), EB (C) and IEA (D) at the indicated concentrations for 24 h. After treatment, cell proteins were then harvested for the Western blot analysis of LC3, GFP and β-actin. Bar chart indicates the relative expression of LC3-II and GFP to β-actin; bars, S.D. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. The full-length Western blot images are showed in Figure S17. (E) ATG7+/+ MEF and ATG7–/– MEF cells transfected with EGFP-HTT74 were treated with EA, EB and IEA at the indicated concentrations for 24 h. After treatment, the cells were fixed and the representative images were captured by fluorescence microscope. Red arrows indicate the cells with formed EGFP-HTT74 inclusion. Magnification: 40×. Scale bar: 50 μm. Bar chart indicates the percentage of cells with EGFP-HTT74 inclusion formation; bars, S.D. ***P ≤ 0.001. The full-length Western blot images are showed in Figure S17.