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. 2020 Mar 2;34:2058738420909041. doi: 10.1177/2058738420909041

Figure 3.

Figure 3.

miR-142-5p directly targeted IGF1. (a) The seed sequence of miR-142-5p targets the WT or MUT 3′UTR of IGF1 mRNA. (b) Luciferase activity of HEK293 cells co-transfected with miR-142-5p or miR-NC and luciferase reporters of IGF1 mRNA 3′UTR-WT or -MUT vector were checked by dual-luciferase reporter assays. (c and d) The influence of inhibition of miR-142-5p level upon NG condition and up-regulation of miR-142-5p level under HG treatment on the expressions of IGF1 mRNA or protein levels were measured by qRT-PCR or Western blot methods, respectively (mean ± SD; **P < 0.01, ***P < 0.001).