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. 2020 Mar 2;34:2058738420909041. doi: 10.1177/2058738420909041

Figure 5.

Figure 5.

Effects of changed miR-142-5p expression along with MEK, AKT, and PI3K inhibitors on HREC proliferation. (a and b) Influences of suppressed miR-142-5p level with PI3K inhibitor LY294002 or MEK inhibitor PD0325901 on the IGF1/IGF1R-mediated signaling pathway and VEGF levels were detected by Western blot method under NG condition. (c) Role of enhanced miR-142-5p level accompanying with AKT agonist SC-79 in the expressions of IGF1/IGF1R-mediated signaling pathway and VEGF levels were measured by Western blot assay to HG treatment. (d) Effects of prohibition the miR-142-5p level meanwhile with treatment of LY294002 or PD0325901 on the HRECs viability were tested by CCK8 assay under NG treatment. (e) Influences of miR-142-5p mimic following with SC-79 treatment on the HRECs proliferation were examined by CCK8 assay upon HG treatment. (f and g) Functions of co-treatment of miR-142-5p inhibitor and PI3K, MEK inhibitor on Ki-67 levels were checked by IF assay, respectively. (h) Effects of co-treated miR-142-5p mimic and AKT activator were measured by IF method under HG condition. Scale bar = 50 µm. (mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001).