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. 2020 Feb 25;11:138. doi: 10.3389/fpls.2020.00138

Figure 2.

Figure 2

Expression profiles of PTI genes in leaves and roots of tomato plants in response to M. hapla J2 with and without attached Microbacterium sp. K6. Defense gene expression of invaded plants relative to not inoculated plants, as determined in cDNA by Real-Time PCR, was normalized for ubiquitin transcripts and M. hapla 18S rRNA in roots, using the 2–ΔΔCt method. Bars show means of log-transformed fold-changes in gene expression, one and three days after J2 inoculation to the roots (dpi). ANOVA by generalized linear models (see Supplement Statistics 2 ) revealed that the attached bacteria K6 (stages: with, without) significantly enhanced defense gene expression overall (P < 0.0001). The factors DPI (stages: 1 dpi, 3 dpi) and TISSUE (stages: root, leaf) had significant effects on gene expression. The effect of attached K6 was more pronounced 3 dpi than 1 dpi (P = 0.002 for the interaction effect K6 * DPI). Error bars represent SE (n = 10 plants).