Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 25;10:222. doi: 10.3389/fonc.2020.00222

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Li, Yuan, Tu, Hu, Li, Yi, Li, Zhao, Cheng, Yu, Jian and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Bioengineered nCAR/miR-34a-5p induces apoptosis in ES cells. (A) The apoptotic profiles were evaluated by flow cytometric analysis after Annexin V-FITC/PI staining. (B) Comparison of the percentages of apoptotic cells with different treatments showed that bioengineered nCAR/miR-34a-5p largely induced apoptosis (P < 0.0001, two-way ANOVA). (C–D) Immunofluorescence assessment of apoptotic biomarker cleaved-casepase-3 in ES A673 cells further confirmed the effect of bioengineered nCAR/miR-34a-5p on apoptosis, which was quantitated as over 100% increase in the number of cleaved-caspase-3-positive cells treated with nCAR/miR-34a-5p, as compared to either control RNA or vehicle treatment (P < 0.01, one-way ANOVA). Values are mean ± SD of triplicate treatments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.