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. 2020 Feb 25;10:222. doi: 10.3389/fonc.2020.00222

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Copyright © 2020 Li, Yuan, Tu, Hu, Li, Yi, Li, Zhao, Cheng, Yu, Jian and Yu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bioengineered nCAR/miR-34a-5p causes a G2 cell cycle arrest in ES cells. (A) Cell cycle profiles were determined by flow cytometry analysis after the cells were stained with propidium iodide for DNA contents. (B) Quantitative measurements showed that nCAR/miR-34a-5p treatment led to a remarkable accumulation of ES A673 cells in G2 phase, while the numbers of ES A673 cells in G0/G1 and S phase were reduced accordingly (P < 0.0001, two-way ANOVA). Values are mean ± SD of triplicate treatments. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.