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Quality assessment of mycobacterial genomic DNA. DNA was extracted from four strains of M. tuberculosis and nine NTM species as indicated using the present protocol and assessed on two distinct 0.8% agarose gels in 1X TAE stained with GelRed, 1 kb Plus ladder (Thermo Scientific), with separation for 90 min at 80 V. Quantities range from 15 to 36 ng per lane. The large DNA migrates with the highest marker band at about 20 kbases.
