Fig. 2.

LDLR^−/− mice on Western diet (WD) represent a model of obesity/hyperlipidemia-induced nonalcoholic steatohepatitis (NASH). Wild-type (WT; open bars) and LDLR^−/− (closed bars) mice were placed on chow or WD for 8 wks. Liver weights (A), hepatic lipid measured by Oil red O staining (B) (×10), and liver triglyceride (TG) concentration (C). D: hepatic H&E staining. Liver gene expression of Tnf (E) and Il10 (F) was measured by real-time RT-PCR, and protein levels of IFNγ were measured by ELISA (G). H: hepatic collagen deposition was measured by histological staining with Sirius red. Liver gene expression of Timp1 (I), and Tgfb1 (J) is shown. K: gene and protein expression of total liver alpha-smooth muscle actin (α-SMA). Data are means ± SE of n = 5–9 mice per group. Data analysis: Two-way ANOVA with Tukey correction for A, C, E, F, I, and J. *Genotype effects and ⁺diet effects. * or ⁺P < 0.05, ** or ⁺⁺P < 0.01, ⁺⁺⁺P < 0.001, ⁺⁺⁺⁺P < 0.0001.