Fig. 4.

Isolated hepatic CD8⁺ T cells express an IL-10 phenotype in obesity/hyperlipidemia-induced nonalcoholic steatohepatitis (NASH). Wild-type (WT) and LDLR^−/− mice were placed on chow or WD for 8 wks. Mice were euthanized and livers collected for isolation of CD8⁺ T cells. Isolated CD8⁺ T cells were stimulated with IgG (control) or CD3/C28 for 72 h. Media was collected and secreted cytokine levels of IL-10, TNFα, Granzyme B, IL-6, IL-2, and IL-4 measured by Luminex. Data are means ± SE of n = 3 mice per group. Data analysis: two-way ANOVA with Tukey correction. *P < 0.05; ** or ⁺⁺P < 0.01.