Fig. 6.

Adoptive transfer of hepatic CD8⁺ T cells from obesity/hyperlipidemia-induced nonalcoholic steatohepatitis (NASH) mice increases alpha-smooth muscle actin (α-SMA) expression. LDLR^−/−;GFP^+/+ mice were placed on Western diet (WD) for 8 wks. Mice were euthanized and hepatic CD8⁺ T cells were isolated from livers. Isolated CD8⁺ T cells were then injected into wild-type (WT) chow-fed mice and recipient mice were euthanized 24 h after injections. A: flow cytometry analysis of hepatic CD8⁺TCRβ⁺GFP⁺ cells. B: Tnf, Il10, Itgam, Itgax gene expression in total liver. C: Tgfb1, Timp1, and Acta2 gene expression in total liver. D: immunofluorescence of liver sections stained with α-SMA (11) and DAPI (blue) at ×40. Data are means ± SE of n = 5–6 mice per group. Data analysis: unpaired Student’s t-test. ****P < 0.0001.