Fig. 7.

CD8⁺ T cells do not regulate liver inflammation or activate hepatic stellate cells (HSCs) under nonobese nonalcoholic steatohepatitis (NASH) conditions. Wild-type (WT) mice were placed on high-fat diet with 0.1% methionine and deficient in choline (HFCD) diet for 10 wks and injected weekly with IgG or anti-CD8 antibody throughout the duration of the diet. Mice were euthanized, and livers were isolated for subsequent analysis. A: flow cytometric analysis and quantification of hepatic CD8⁺ and CD4⁺ T cells. B: gene expression in total liver. C: hepatic collagen deposition was measured by histological staining with Sirius red. Data are means ± SE of n = 10–12 mice per group. Data analysis: unpaired Student’s t-test. *P < 0.05.