Fig. 9.

Effect of supramaximal dichlorophene. Dichlorophene is an antagonist of the Ano1 anion channel and blocks myenteric interstitial cell of Cajal oscillations. When a supramaximal concentration of dichlorophene (20 μM) was added to the bath in one go a stripe pattern was seen (center) before contractions disappeared almost completely (right). Times from dichlorophene addition are indicated at top. The intestine significantly lengthened in response to dichlorophene, as indicated by the shift in the position of mesenteric/Peyer's diameter markers (arrows). Thus we had to pull the intestine out straight (pull one end’s cannula further down the kit-kat) and so could not map the intestine continuously to register all transitional changes in the motor pattern. Female C57BL/6J, perfused lumen, no carbenoxolone. Representative of 4 experiments.