Table 5.
Fold changes in NHE3 and PTGS2 mRNA expression in polarized Caco-2 cell monolayers after 24-h exposure to IL-1α
| Concentration of Recombinant Human IL-1α |
||||
|---|---|---|---|---|
| Gene Name | Control | 10 ng/ml | 100 ng/ml | 500 ng/ml |
| NHE3 | 1.07 ± 0.18a | 1.48 ± 0.32a | 1.60 ± 0.49a | 1.28 ± 0.29a |
| PTGS2 | 1.00 ± 0.05a | 2.00 ± 0.24b | 2.38 ± 0.34b | 2.02 ± 0.20b |
Data are means ± SE of fold differences in gene expression measured by quantitative RT-PCR; n = 6. NHE3, Na+/H+ exchanger; PTGS2, prostaglandin-endoperoxide synthase 2. Significantly different values are marked in bold.
Each gene marked with a different superscript differs by at least P < 0.05 compared with control.