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. 2019 Nov 25;318(2):G265–G276. doi: 10.1152/ajpgi.00218.2019

Fig. 3.

Fig. 3.

Pancreatic tumor development in Pdx1Cre;LSL-KrasG12D mice fed alcohol or Western diet or administered cerulein. A: Pdx1Cre;LSL-KrasG12D mice were fed a Lieber-DeCarli diet containing 3.5% alcohol or a Western diet for 5 mo. To induce pancreatitis, mice were injected with cerulein every month from 1st to 4th month. B–D: pancreas sections were stained with hematoxylin and eosin (H&E) (top), Sirius red (middle), and KRT19 antibodies (bottom). B and C: alcohol (Alc) or Western diet (WD) promotes pancreatic intraepithelial neoplasia (PanIN)-3 (arrowheads) and pancreatic ductal adenocarcinoma (PDAC; arrows). D: cerulein administration induces pancreatic intraepithelial neoplasia (PanIN)-3 (arrowheads) but not PDAC. E: H&E staining of the pancreata collected from control mice injected PBS or cerulein (CR). CR treatment induces acute pancreatitis. The control mice fed Alc or WD did not show injury in the pancreata. Bar = 20 μm.