Fig. 6.

Phosphorylation of acetyl-CoA carboxylase (ACC) at serine 79 (p-ACC Ser⁷⁹). Protein content of cytosolic p-ACC Ser⁷⁹ before (rest), immediately postexercise (+0 h), and 3 h (+3 h) after a single session of high-intensity interval exercise (HIIE) performed at the same absolute intensity before (pre-HVT) and after (post-HVT) 40 sessions of twice-daily high-volume, high-intensity interval training (HVT) in the vastus lateralis muscle of young healthy men (n = 9). ○ (Pre-HVT) and ◇ (post-HVT) represent individual values; white (pre-HVT) and gray (post-HVT) bars represent mean values. #Main effect of exercise (P < 0.05) vs. rest; *P < 0.05 vs. rest in the same group by 2-way ANOVA with repeated measures, followed by Tukey’s honestly significant difference post hoc test or by preplanned paired t-test for rest values between trials. To more clearly depict fold changes in postexercise values from potentially different rest values in the untrained and trained states, an inset has been added; error bars represent the SE. AU, arbitrary units.