Fig. 7.

p53 and Plant homeodomain finger-containing protein 20 (PHF20) protein. Protein content of nuclear (A) and cytosolic (B) p53, nuclear (C) and cytosolic (D) PHF20, and nuclear (E) and cytosolic (F) p-p53 Ser¹⁵ assessed before (rest), immediately postexercise (+0 h), and 3 h (+3 h) after a single session of high-intensity interval exercise (HIIE) performed at the same absolute intensity before (pre-HVT) and after (post-HVT) 40 sessions of twice-daily high-volume, high-intensity interval training (HVT) in the vastus lateralis muscle of young healthy men (n = 9). ○ (Pre-HVT) and ◇ (post-HVT) represent individual values; white (pre-HVT) and gray (post-HVT) bars represent mean values. #Main effect of exercise (P < 0.05) vs. rest; *P < 0.05 vs. rest of the same group; †P < 0.05 vs. the same time point of post-HVT trial by 2-way ANOVA with repeated measures, followed by Tukey’s honestly significant difference post hoc test or by preplanned paired t-test for rest values between trials. To more clearly depict fold changes in postexercise values from potentially different rest values in the untrained and trained states, an inset has been added to each graph (note: significant differences between trained and untrained values at rest are not reported in the insets, as both of these values are normalized to 1); the error bars represent SE. AU, arbitrary units.