Table 2.
mRNA content measured in whole tissue of cyt c, GCN5, HSP70, NRF-1 and -2, PHF20, PPARα, PPARδ, PPARγ, SIRT1, and TFAM measured immediately postexercise (+0 h) and 3 h (+3 h) after a single session of pre-HVT and post-HVT in the vastus lateralis muscle of young healthy men
| Gene (Interaction) | Pre-HVT | Post-HVT |
|---|---|---|
| cyt c (P = 0.079) | ||
| Rest | 2.06 ± 0.89 | 2.79 ± 1.55 |
| +0 h# | 1.63 ± 0.44 | 1.46 ± 0.67 |
| +3 h | 1.70 ± 0.64 | 2.48 ± 1.37 |
| GCN5 (P = 0.718) | ||
| Rest | 0.05 ± 0.06 | 0.09 ± 0.15 |
| +0 h | 0.06 ± 0.04 | 0.08 ± 0.07 |
| +3 h | 0.06 ± 0.08 | 0.13 ± 0.14 |
| HSP70 (P = 0.021‡) | ||
| Rest | 1.15 ± 0.84 | 1.98 ± 1.43 |
| +0 h | 4.49 ± 3.01*† | 2.00 ± 0.84 |
| +3 h | 3.87 ± 1.81* | 2.63 ± 1.11 |
| NRF-1 (P = 0.118) | ||
| Rest | 0.14 ± 0.08 | 0.13 ± 0.06 |
| +0 h | 0.19 ± 0.09 | 0.14 ± 0.04 |
| +3 h | 0.15 ± 0.05 | 0.16 ± 0.05 |
| NRF-2 (P = 0.962) | ||
| Rest | 0.23 ± 0.07 | 0.21 ± 0.06 |
| +0 h | 0.23 ± 0.09 | 0.19 ± 0.05 |
| +3 h | 0.28 ± 0.13 | 0.26 ± 0.15 |
| PHF20 (P = 0.279) | ||
| Rest | 0.34 ± 0.08 | 0.27 ± 0.04 |
| +0 h | 0.31 ± 0.08 | 0.24 ± 0.05 |
| +3 h | 0.31 ± 0.09 | 0.29 ± 0.09 |
| PPARα (P = 0.005‡) | ||
| Rest | 0.43 ± 0.20 | 0.61 ± 0.38 |
| +0 h | 0.27 ± 0.19 | 0.31 ± 0.08 |
| +3 h | 1.66 ± 0.91*† | 0.87 ± 0.40 |
| PPARδ (P = 0.766) | ||
| Rest | 0.00 ± 0.00 | 0.01 ± 0.03 |
| +0 h | 0.01 ± 0.01 | 0.01 ± 0.02 |
| +3 h | 0.02 ± 0.04 | 0.02 ± 0.02 |
| PPARγ (P = 0.096) | ||
| Rest | 0.06 ± 0.02† | 0.08 ± 0.03 |
| +0 h | 0.04 ± 0.02 | 0.05 ± 0.02 |
| +3 h | 0.04 ± 0.02 | 0.11 ± 0.11 |
| SIRT1 (P = 0.685) | ||
| Rest | 0.06 ± 0.03 | 0.07 ± 0.04 |
| +0 h# | 0.05 ± 0.03 | 0.05 ± 0.02 |
| +3 h | 0.07 ± 0.02 | 0.06 ± 0.04 |
| TFAM (P = 0.953) | ||
| Rest | 0.48 ± 0.14 | 0.44 ± 0.09 |
| +0 h | 0.40 ± 0.11 | 0.37 ± 0.08 |
| +3 h | 0.47 ± 0.13 | 0.44 ± 0.12 |
All values are means ± SD; n = 9 for nuclear respiratory factor (NRF)-1; n = 7 for plant homeodomain finger-containing protein 20 (PHF20); n = 8 for all other genes. Cyt c, cytochrome c; GCN5, histone acetyltransferase KAT2A; HSP70, heat shock 70 kDa protein 1A; PPARα, peroxisome proliferator-activated receptor-α; PPARδ, peroxisome proliferator-activated receptor-δ; PPARγ, peroxisome proliferator-activated receptor-γ; pre- and post-HVT; high-intensity interval exercise performed at the same absolute intensity before (pre-HVT) and after (post-HVT) 40 sessions of twice-daily high-volume high-intensity interval training (HVT); SIRT1, NAD-dependent protein deacetylase sirtuin-1; TFAM, mitochondrial transcription factor A. Values are expressed relative to TATA-binging protein, glyceraldehyde 3-phosphate dehydrogenase, and β-actin housekeeping genes.
Interaction effect (P < 0.05);
main effect of exercise (P < 0.05) vs. rest;
P < 0.05 vs. rest in the same group;
P < 0.05 vs. same time point of post-HVT trial by 2-way ANOVA with repeated measures, followed by Tukey’s honestly significant difference post hoc test or preplanned paired t-test for rest values between trials.