A: phosphorylated AMPKαThr172/AMPKα immediately postexercise. B: phosphorylated ACCSer79/212/ACC immediately postexercise. C: phosphorylated AMPKαThr172/AMPKα 3 h postexercise. D: phosphorylated ACCSer79/212/ACC 3 h postexercise. IPEX data were analyzed by one-way ANOVA and 3hPEX data were analyzed by two-way ANOVA. *P < 0.005, HFD-IPEX greater than both other groups. †P < 0.01, main effect of 3hPEX vs. both LFD-SED and HFD-SED. Values are expressed as means ± SEM; n = 8–17/group. HFD, high-fat diet; LFD, low-fat diet; SED, sedentary; IPEX, immediately postexercise; 3hPEX, 3 h postexercise.