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A–D: phosphorylated AktThr308/Akt, Akt2Thr309/Akt2, AktSer473/Akt, and Akt2Ser474/Akt2 at 3 h postexercise. Data were analyzed by two-way ANOVA. *P < 0.001, insulin vs. no insulin. †P < 0.01, significantly different from both other treatment groups within insulin-treated muscles. ‡P < 0.05, significantly different from LFD-SED within insulin-treated muscles. Values are expressed as means ± SEM; n = 10/group. HFD, high-fat diet; LFD, low-fat diet; SED, sedentary; 3hPEX, 3 h postexercise.
