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. 2020 Jan 16;128(2):286–295. doi: 10.1152/japplphysiol.00332.2019

Fig. 5.

Fig. 5.

Abundance of stress response protein2 (Sestrin2)-GAP activity toward RAGs (GATOR)2, RAS-related GTP-binding protein (Rag)A-mechanistic target of rapamycin (mTOR), and RagC-mTOR complexes in longissimus dorsi muscle of neonatal pigs fed low-protein (LP) diets supplemented with β-hydroxy-β-methylbutyrate (HMB) or leucine (Leu) or a high-protein (HP) diet. A: Sestrin2-GATOR2 complex abundance (study 1). B: Sestrin2-GATOR2 complex abundance (study 2). C: RagA-mTOR complex abundance (study 1). D: RagA-mTOR complex abundance (study 2). E: RagC-mTOR complex abundance (study 1). F: RagC-mTOR complex abundance (study 2). AU, arbitrary units; HMB4, HMB40, and HMB80, 4, 40, and 80 μmol HMB·kg body wt−1·day−1. On the blot images, white lines between bands indicate where images from the same blots were spliced to adjust sample order on the membrane for presentation. Statistical analyses were performed with 1-way ANOVA followed by Tukey’s test. Values are means ± SE; n = 7–10 pigs. Means with different letters differ at P < 0.05.