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Antibodies used in immunoblotting
| Antibody Target | Primary Ab Supplier | Ab Host | Dilution | Secondary Ab Supplier | Ab Host | Dilution |
|---|---|---|---|---|---|---|
| α-ENaC | StressMarq (SPC-403D) | Rabbit | 1:1,000 | Invitrogen (SA5-10036) |
Goat | 1:1,000 |
| β-ENaC | ProSci (Poway, CA) (21, 34) | Rabbit | 1:2,000 | Invitrogen (SA5-10036) |
Goat | 1:1,000 |
| γ-ENaC | StressMarq (SPC-405) | Rabbit | 1:1,000 | Invitrogen (SA5-10036) |
Goat | 1:1,000 |
| Actin | Sigma (A1978) | Mouse | 1:20,000 | Invitrogen (A21057) |
Goat | 1:10,000 |
ENaC, epithelial sodium channel.
