Fig. 3.

Angiotensin II type 1 receptor agonistic autoantibody (AT₁-AA) inhibition reduced mitochondrial oxidative stress (mtROS) production in reduced uterine perfusion pressure (RUPP) kidney. Respiration rates were measured in isolated mitochondria using the Oxygraph 2K, and mtROS (H₂O₂) was measured using Amplex red assay with a fluorescent microplate reader. State 3 respiration (A) and maximal respiration (B) in RUPPs treated with AT₁-AA inhibitory peptide [′n7AAc′; n = 9 (A) and 6 (B)] vs. RUPPs [n = 6 (A) and 6 (B)]. Furthermore, AT₁-AA inhibition significantly reduced mtROS production in RUPPs treated with ′n7AAc′ (n = 3) vs. RUPPs (n = 4) (C). Data are presented as means ± SE. *P < 0.05 vs. RUPP, Student’s t-test.