Skip to main content
. 2019 Nov 13;318(2):C242–C252. doi: 10.1152/ajpcell.00123.2019

Fig. 4.

Fig. 4.

Mitochondrial-specific autophagy related protein expression and mitochondria localized autophagy response after freeze injury. A: semiquantitative analysis of dynamin-related protein (Drp1), BCL1 interacting protein 3 (Bnip-3), Pink, and Parkin protein expression 7 days after freeze injury and representative immunoblot images. (n = 7) Blots are normalized to total protein as a loading control. Differences in protein expression after injury were detected by RM ANOVA between uninjured and injured limbs. *Significantly different from uninjured limb. B: semiquantitative analysis of microtubule-associated protein light chain B1 (LC3)II expression and COXIV expression in cytosolic and mitochondrial-enriched fractions from mice 7 days after freeze injury followed by representative immunoblot images (n = 8). Blots are normalized to total protein as a loading control and presented as relative expression to uninjured cytosol fraction. Differences were analyzed by two-way RM ANOVA with the repeated measures being the injured vs. uninjured contralateral control limb and the other factor being fraction (mitochondria-enriched or cytosolic). *Significantly different from all other groups. Data are presented as means ± SD. DRP1; dynamin-related protein.