Fig. 3.

Soleus (SOL) fiber myofilament protein isoform expression. A: silver stain of representative single SOL fiber homogenates demonstrating two classes of SOL fibers that express either slow troponin (Tn)T, slow TnI, and slow myosin heavy chain (MHC)-I (Slow SOL) or fast TnT, fast TnI, and mixed slow MHC-I and fast MHC-IIa and MHC-IIx (Fast SOL). B–D: Western blots of representative single Slow SOL and Fast SOL fiber homogenates with mAb CT3 (slow skeletal muscle TnT specific) alone or in combination with mAb T12 (fast skeletal muscle TnT specific) (B), mAb TnI-1 (fast and slow skeletal muscle TnI) (C), and silver stain or FA2 (type I slow MHC specific) (D). Mr Mkr, molecular marker.