Fig. 7.

PKCβ and mitochondrial reactive oxygen species facilitate enhanced basal tone in isolated pulmonary arteries from chronic hypoxia (CH) neonates. Experiments were conducted in the presence of the mitochondria-targeted $O_2^{-}$ scavenger (2-(2,2,6,6-tetramethylpiperidin-1-oxyl-4-ylamino)-2-oxoethyl)triphenylphosphonium chloride (MitoTEMPO, 200 μM; A), the coenzyme Q10 analog mitoquinone mesylate (MitoQ, 1 μM; B), the PKCβ inhibitor LY-333,531 (LY, 10 nM; C), or vehicle. Basal tone (% vasoconstriction) is expressed as % maximally dilated (Ca²⁺ free) inner diameter. All experiments were conducted in the presence of N^ω-nitro-l-arginine (300 μM). Values are means ± SE; n = 5–10 rats/group (indicated in bars). *P < 0.05 vs. control, ^#P < 0.05 vs. vehicle, analyzed by 2-way ANOVA followed by Student-Newman-Keuls post hoc test.