Fig. 2.

Sox6 is essential for cAMP induction of renin expression in vitro. Renal CD44⁺ mesenchymal stromal cells (MSCs) were isolated from adult wild-type mice. Quantitative RT-PCR analysis of renin and Sox6 expression after 3-isobutyl-1-methylxanthine and forskolin (I&F) treatment was performed after 7 days of treatment. Data are presented as means ± SE; n = 4. P values were calculated with an unpaired t test. *P < 0.05; ***P < 0.001. A: renin. B: Sox6. Renal CD44⁺ MSCs were isolated from C57BL6 Ren1c yellow fluorescent protein (YFP) adult mice and cultured in growth medium for three to five passages before use, and YFP expression was used as a surrogate for renin expression. C: quantitative RT-PCR analysis of Sox6 knockdown in MSCs. Data are presented as means ± SE of three independent experiments. P values were calculated with an unpaired t test. *P < 0.05. D: downregulation of Sox6 affects the differentiation of renal MSCs to renin-expressing cells. Data are presented as means ± SE of three independent experiments. P values were calculated using an unpaired t test. **P < 0.01 comparing Sox6 shRNA with control shRNA or cAMP-responsive element modulator (Crem) shRNA with control shRNA.