Fig. 1.

Diabetes increases kidney asymmetric dimethylarginine (ADMA) and reduces kidney dimethylarginine dimethylaminohydrolase (DDAH) activity and DDAH-1 expression. A: kidney ADMA levels were determined by ELISA. B: DDAH activity was determined as described in materials and methods. C and D: levels of DDAH-1 and DDAH-2 protein in normal and diabetic mouse kidneys were determined by Western blot analysis and normalized to GAPDH. Western blots are representative of 5 mice/group. Data are presented as means ± SE. *P < 0.05 compared with normal.