Fig. 4.

Administration of adenovirus expressing dimethylarginine dimethylaminohydrolase-1 (Ad-h-DDAH-1) increases kidney endothelial nitric oxide synthase (NOS3) mRNA and reduces urinary thiobarbituric acid-reactive substances (TBARS) in diabetic mice. A: total RNA was isolated from mouse kidneys, and RT-PCR was performed to determine NOS3 mRNA levels normalized to 18S rRNA. B: mouse 24-h urine was collected for the measurement of TBARS. Data are presented as means ± SE; n = 5–10 mice/group. *P < 0.05 and **P < 0.001 compared with normal; #P < 0.005 compared with adenovirus expressing green fluorescent protein (Ad-GFP)-treated diabetic mice.