Quantification of internalized fluorescent labeling after pulse-chase experiments in the presence of 10 μM CytD,10 μM LatA or 0.5 μM Jas (A), 5 μM filipin (B), 15 μM PitStop 2 (C) or 80 μM dynasore (D). The number of internalized fluorescent dots was relativized to control (-, DMSO). Inhibitors were added during pulse to prevent possible deleterious effects on cortical exocytosis. The total number of evaluated eggs for each group is shown in brackets. *p<0.05, **p<0.01.