Figure 4. Compensatory endocytosis is impaired in A23187-activated eggs.

Zona-free eggs were incubated with 10 μM Ca²⁺ ionophore A23187 in Ca²⁺/Mg²⁺-free medium for 5 min, thoroughly washed, incubated for additional 10 min in complete medium, and pulse-chase experiments were performed. Cortical exocytosis was measured as the fluorescent intensity in the membrane after pulse (A) and the relative number of fluorescent dots per confocal plane after chase was determined (B). AU: Arbitrary Units. The total number of evaluated eggs for each group is shown in brackets. *p<0.05, **p<0.01, ***p<0.001.