Fig. 2.

Bone morphogenetic protein receptor type 2 (BMPR2) mutation in cardiomyocytes predominately enhances the expression of proteins influencing insulin sensitivity and blunts their response to insulin. A: Western blot gels and bar graph for phosphorylated (p) Akt^Ser473 in mutant and control at baseline and following insulin stimulation. B: Western blot gels and bar graph for p-AMPK^Thr172 in mutant and control at baseline and following insulin stimulation. C: Western blot gels and bar graph for p-acetyl-CoA carboxylase (ACC)^Ser79/pACC^Ser221 in mutant and control at baseline and following insulin stimulation. D: Western blot gels and bar graph for pACC^Ser79 in mutant and control at baseline and following insulin stimulation. Open bars, control (n = 3–5); light gray bars, mutant 1 (M1, n = 3–5); dark gray bars, mutant 2 (M2, n = 3–5). P < 0.05, protein expression in mutant cells (M1 or M2) vs. controls (*), following insulin stimulation (#), and M1 vs. M2 cells ($). Also see Supplemental Fig. S2. Statistical test used was t test.