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. 2019 Dec 18;318(2):L429–L441. doi: 10.1152/ajplung.00555.2018

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Fig. 3. — Immunohistochemical analysis of phosphorylated (p)-Akt^Ser473, p-AMPK^Thr172, and p-acetyl-CoA carboxylase (ACC)^Ser79 in human right ventricular (RV) tissue from control and pulmonary arterial hypertension (PAH) patients. A: pAkt^Ser473 staining the RV tissue is in brown and nuclei were counterstained with hematoxylin. Magnification ×400. Bar graphs represent semiquantitative analysis of brown staining using Fiji software [control (n = 4), PAH (n = 6)]. B: pAMPK^Thr172 staining the RV tissue is in brown and nuclei were counterstained with hematoxylin. Magnification ×400. Bar graphs represent semiquantitative analysis of brown staining using Fiji software [control (n = 7), PAH (n = 8)]. C: pACC^Ser79 staining the RV tissue is in brown and nuclei were counterstained with hematoxylin. Magnification ×400. Bar graphs represent semiquantitative analysis of brown staining using Fiji software [control (n = 7), PAH (n = 8)]. *P < 0.05. Statistical test used was t test.