Fig. 5.

Bone morphogenetic protein receptor type 2 (BMPR2) mutation increases CD36 protein expression and palmitate uptake, and insulin-stimulated palmitate uptake is dependent on the type of BMPR2 mutation. A: immunofluorescence staining of CD36 (in red) and nucleus (in blue) in control, mutant 1 (M1), and mutant 2 (M2) cells. Magnification ×400. B: semiquantitative analysis of CD36 immunofluorescence intensity in control and mutant cells (*P < 0.05). C: Western blot gels and bar graphs representing CD36 protein in mutant and control at baseline and following insulin stimulation (n = 6). P < 0.05, protein expression in mutant cells vs. controls (*) and following insulin stimulation (#). D: [¹⁴C]palmitate uptake in control and mutant cells following insulin stimulation for 60 min (n = 6). *P < 0.0001, fold increase in palmitate uptake in mutant cells compared with control. #P < 0.05, fold increase in palmitate uptake in mutant cells following 60 min of insulin stimulation. Statistical test used was t test.