Fig. 6.
Bone morphogenetic protein receptor type 2 (BMPR2) mutation increases milk fat globule-EGF factor 8 (MFGE8) gene expression in cultured cardiomyocytes. A: principal component analysis for control (open circles) and mutant (black circles) cells. B: heat map of genes involved in insulin sensitivity. C: MFGE8 mRNA expression in control and mutant cells. Open bars, control; dark gray bars, mutant 1 (M1); light gray bars, mutant 2 (M2). *P < 0.05, MFGE8 gene expression in M1 and M2 vs. control (C). D: MFGE8 mRNA expression in right ventricular (RV) tissue from BMPR2R899X mice compared with control mice. Open bars, control; black bars, mutant and Rosa26R899X. Also see Supplemental Fig. S3. Statistical test used was t test. *P < 0.05, MFGE8 gene expression in BMPR2 mutant mouse RV vs. control mouse RV.