Fig. 7.

Budesonide augments formoterol-mediated attenuation of carbachol (Cch)-induced phosphorylation of myosin light chain. Human airway smooth muscle (HASM) cells were treated with Cch (25 μM, 15 min), then treated simultaneously with formoterol (Form; 10 pM–10 nM, 5 min) and budesonide (Bud; 1 μM, 5 min). Phosphorylation of myosin light chain was assessed by immunoblot, using total myosin light chain as a loading control (A). Data are expressed as fold compared with control (B) and are representative of five separate HASM donors (*P < 0.05).